blue-white screen
A screening technique for the rapid detection of recombinant bacteria in vector-based…A screening technique for the rapid detection of recombinant bacteria in vector-based molecular cloning experiments. DNA of interest is ligated into a vector, which is then inserted into a host cell; these are grown in the presence of X-gal. Cells transformed with vectors containing recombinant DNA will produce white colonies; cells transformed with non-recombinant plasmids (i.e. only the vector) grow into blue colonies.
Meaning
1 sensesA screening technique for the rapid detection of recombinant bacteria in vector-based molecular cloning experiments. DNA of interest is ligated into a vector, which is then inserted into a host cell; these are grown in the presence of X-gal. Cells transformed with vectors containing recombinant DNA will produce white colonies; cells transformed with non-recombinant plasmids (i.e. only the vector) grow into blue colonies.
Forms
plural: blue-white screens| Singular | blue-white screen |
|---|---|
| Plural | blue-white screens |
regular